MatriWell™ are cell culture inserts containing a hydrogel with tissue-specific extracellular matrix proteins, designed for fabricating 3D epithelial barriers in vitro. When cells are cultured on MatriWell™ , they are exposed to an air interface on their top and a near-liquid interface on their bottom, an "air-gel interface". This setup mimics the exact microenvironment cells would find in the native tissue.